The main purpose is to produce healthy plants that are free of virus diseases, particularly banana bunchy top and banana mosaic. The plantlets also have the advantages that they are more uniform than suckers, and the adult plants can be harvested over a shorter period.
Existing problems were discussed, aiming to provide scientific references for promoting tissue culture and genetic transformation of kenaf.
Kenaf originates in Africa and owns extensive distribution in the world, mainly concentrating in tropical and subtropical zones, Asia and Latin America. Kenaf is characteristic of short growth cycle, tall strong plants, high stress resistance, soft bast fiber, strong tensile force of fiber, high fiber yield, good hygroscopicity and quick water dispersal.
Biological yield of kenaf is about times that of forest and CO2 assimilation capacity is about times that of trees [ 1 ].
Full-pole reproduced pulp quality is equal to that prepared by broadleaf forest. It is widely accepted as a new raw material for papermaking and is highly appreciated in developed countries.
Wood pulp has lost advantages in papermaking due to massive deforestation behaviors and intensifying environmental problems e. Kenaf is viewed as high-quality papermaking raw materials in view of fast growth, high yield, suitable growth in river and lake bottomlands and compatibility with grains and cottons.
Rapid development of kenaf production is of important significance to protect forest resources and develop national economy. In addition, kenaf is an important industrial Micropropagation of kenaf thesis of traditional hemp spinning industry.
Great changes on production of bast fiber crop and its scientific research have taken place. These are consequences of extensive use of chemical fiber materials since s. They put forward higher and higher requirements on quality of life.
Additionally, kenaf products are environmental-friendly. For example, the Japanese Dongli wallpaper made of kenaf uses aqueous solvent which is safe to human health.
Compared to other wallpapers in the market, Japanese Dongli wallpaper contains no harmful volatile organic compounds like methylbenzene and xylene. Moreover, it is degradable in soil. Therefore, developing kenaf production can be used as one of countermeasures to protect forest resources and prevent air pollution and global warming.
Textile export can be increased by hemp cotton and hemp-fiber blended products. Developing kenaf production is an important way to solve increasing demands of natural fiber. Germplasm innovation of kenaf is a crucial strategy to realize this goal.
Therefore, developing kenaf varieties with high quality and high yield is an effective guarantee to satisfy natural fiber demands and promote harmonious development of agricultural ecology.
Plant tissue culture has been developed for over years and has become an indispensable technology in bioscience. It plays an important role in crop breeding and genetic transformation.
So far, more than varieties of plants have been regenerated successfully through cell tissue culture [ 2 ]. Great progress in tissue culture research e.
Anther pollen and ovary culture are conducive to accelerate isozygoty of crop offsprings, combine multiple properties quickly, shorten breeding process, simplify the breeding procedure and realize rapid propagation of varieties [ 34 ].
Breeding methods are improved by developing tissue culture technologies. For instance, aneuploid and polyploidy plants are gained through chromosome variation in anther and cell tissue culture, and then used for breeding of crops by chromosome engineering.
Distant hybrid plants were acquired by distant hybrid embryo culture, thus enabling to screen new varieties or distant hybrid species of new materials for breeding. Intraspecific and interspecific somatic cell hybrid plants were acquired by protoplast fusion. The expected somatic cell hybrid of new species was gained.
Transgenic plant was cultured by using protoplast as the target gene receptor, so the expected transgenesis species were screened. With tissue culture technology, existing conventional breeding technique can be reformed and new breeding technique will be developed.
These are conducive to modernization and high-efficiency of crop improvement.
Transgenic breeding is the most effective way for new variety breeding and variety improvement. Scholars attach high attention to transgenic technology to improve breeding of kenaf species and resistance to external environmental factors. In this paper, existing research on tissue culturing and genetic transformation of kenaf were reviewed systematically.
Research conclusions provide some scientific references for further development of tissue culture and genetic transformation of kenaf. Since the regeneration of plants does not requirecallus culture, it shortens the expanding reproduction period and reduces mutations in tissue cultures [ 5 ].
They cultivated multiple shoots, accompanied with basically same shooting rates of different test varieties. They reported the highest shooting rate Besides, adding exogenous hormone in MS culture media will cause dwarf of adventitious bud and easy formation of callus.
High-concentration of 6-BA and NAA is disadvantageous to induction of regeneration bud and shooting, but can form abundant callus.
They also achieved protoplast regeneration plants of the grass family for the first time.SUPERVISION SHEET PHYSOLOGICAL STUDIES ON MICROPROPAGATION OF JACKFRUIT (Artocarpus heterophyllus L.)ashio-midori.com Thesis In Agric.
Sci. (Plant Physiology) By NAGLAA ABDALLAH ABDELSALAM. Thesis on micropropagation of plants SlideShare The high cellulose and lignin content along with insufficient Nitrogen supplies creates depletion problems which can severely restrict plant establishment.
Microbial Contaminants in Plant Tissue Culture Propagation Springer Figure. Inside. Micropropagation TERI University-Ph.D.
Thesis, Micropropagation of Swertia chirayita Introduction In vitro culture is an efficient method for ex situ conservation of plant biodiversity and multiplication of the endangered species.
Kenaf is viewed as high-quality papermaking raw materials in view of fast growth, high yield, suitable growth in river and lake bottomlands and compatibility with grains and cottons. Rapid development of kenaf production is of important significance to protect forest resources and develop national economy.
Kenaf (Hibiscus cannabinus) is an annual non-wood plant which has shown great potential as an alternative source of papermaking fiber. The purpose of this research was to investigate the suitability of Malaysian cultivated kenaf fibers in the production of high quality printing paper.
A thesis submitted in fulfilment of the requirements for the degree of Master of Science Faculty of Agro Based Industry UNIVERSITI MALAYSIA KELANTAN Schematic sketch of micropropagation protocol for kenaf var.
KB6 82 REFERENCES 83 APPENDIX 92 Appendix A-Table A.1 92 Appendix B-Figure B.1 93 -Figure B.2 .